Figure 2

Overexpression of miR-98 prevents cardiomyocyte apoptosis in response to H₂O₂. NRVCs were transfected with NC (negative control), miR-98 mimic (miR-98 mim), miR-98 inhibitor (miR-98 inh), and miR-98 mimic + inhibitor (miR-98 mim&inh) and then treated with H₂O₂ (100 μM) for 4 h. (A) MTT assay suggested that miR-98 mimic restored cell viability of NRVCs treated with 100 μM H₂O₂ for 4 h. n = 8. (B) Statistical results of TUNEL-positive cells per field indicated that miR-98 mimic suppressed H₂O₂ treatment-induced cell apoptosis. n = 6. (C) Representative images of TUNEL staining of NRVCs for DNA defragmentation showing the apoptotic cells (nucleus stained in blue with DAPI and apoptotic cells stained in green). (D) The representative images of flow cytometry using Annexin V-FITC and PI staining. (E)-(G) Statistical analysis of apoptotic, survival and necrosis ratio of the flow cytometry data. n = 4; **P < 0.01 versus control; ^## P < 0.01 versus H₂O₂ + NC cells.