Figure 2 | Scientific Reports

Figure 2

From: Allostimulatory capacity of conditionally immortalized proximal tubule cell lines for bioartificial kidney application

Figure 2

Release of soluble HLA (sHLA) class I antigens by ciPTEC. Secretion of sHLA class I molecules in cell culture supernatant following 48 h stimulation with IFN-γ (300 ng/ml), LPS (10 µg/ml) and indoxyl sulfate (IS) (1 mM) by (a) ciPTEC-U and (b) ciPTEC-T1, measured using ELISA assay and expressed as pg/ml (mean ± SEM). Two independent experiments were performed in duplicate. ***p < 0.001 compared to untreated cells (One-way ANOVA, Dunnett’s multiple comparison test).

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