Figure 3
Expression of co-stimulatory molecules in ciPTEC. (a) Representative histograms from flow cytometric analysis of HLA-DR, CD40, CD80 and CD86 in ciPTEC-U, -T1 and monocytes (used here as a cell type positive for all four surface markers evaluated). In all histogram plots black color represents blank (unstained cells) and red color represents basal expression of either HLA-DR, CD40, CD80 or CD86. (b) Quantification of HLA-DR and CD40 expression in ciPTEC cell lines, expressed as % of positive cells ± SEM and MFI ± SEM respectively. Three independent experiments were performed. *p < 0.05, ***p < 0.001, compared to untreated control (One-way ANOVA, Dunnett’s multiple comparison test).
