Figure 8
HOla promotes the drug transport into the nuclear compartment. HCC1937 were treated with increasing concentration of Ola or HOla (1 and 5 nM) for 72 h. At the end of incubation, cells were collected by tripsinization and subjected to cell fractionation to obtain crude nuclei and cytoplasmic extract. The protein content of each fraction was assessed by Bradford assay and used for normalization. Samples were processed for HPLC-MS quantification of Ola. Reported values are the mean of 2 samples ± s.e. Statistical significance HOla vs. Ola *P < 0.05 (Student’s t-test).
