Figure 2
From: Rapid de novo assembly of the European eel genome from nanopore sequencing reads
Assembly strategy. (a) Stages in the TULIP assembly of the European eel genome. (b) Graph construction based on long read alignments to short seeds. Seeds are included in the graph as nodes if they align adjacent to each other to a long read. The apparent distance between the seeds is included as an edge property, as is the amount of evidence (i.e. number of alignments supporting the connection). (c) The initial seed graph based on alignments contains ambiguities, caused by missed alignments, repetitive seed sequences and spurious alignments. These are removed during the initial layout process, resulting in linear scaffolds. Where possible, these scaffolds are subsequently linked by further unambiguous long-distance co-alignments to long reads.
