Figure 4
Ca2+ entry through nvTRPM2 induced by 2-APB in calcium-imaging experiments. (a) Representative traces from cells that either did or did not express nvTRPM2 after application of 2-APB (1 mM) to the bath. (b) Traces from some mock-transfected cells that showed the highest levels of responses that cannot be attributed to 2-APB. (c) 2-APB induced Ca2+ entry occurs exclusively in cells transfected with nvTRPM2 and requires 1 mM 2-APB. Left: Comparison of nvTRPM2-transfected cells with mock-transfected and hTRPM2-transfected cells (summary). Right: Stimulation of hTRPM2 (in the absence of 2-APB) was still possible with H2O2 (10 mM), demonstrating the functional expression of the channel. Note that the increase of [Ca2+]i induced by 2-APB in nvTRPM2 is comparable to that of H2O2 in hTRPM2. Asterisks indicates significant differences (*** P < 0.001; Student’s t-test, n = 5–15). Error bars are s.e.
