Figure 6
The role of PIK3R1Met326Ile on cell process. (A) Sequencing of PIK3R1 in U87MG. (B) Western blotting of CCN1 and PIK3R1 protein in U87MG cells transfected with the indicated plasmids. Full-length blots are presented in Supplementary Figure S2. (C) WST-1 assays for cell proliferation in U87MG cells transfected with the indicated plasmids (n = 5). (D,E) Migration assays in U87MG cells transfected with the indicated plasmids (n = 3). Quantification of migrated cells are shown on the graph in D. Representative images of each cell transfection group are shown in E. Statistical significance was calculated by ANOVA with Dunnett’s post hoc test. *p < 0.05. Data in C and D are shown as the mean ± SEM. Scale bar = 100 μm.
