Figure 5

Alizarin Red staining and ALP assay of BMMSCs cultured in ICA-SF/PLCL nanofibrous membrane–released medium. (A) Mineralized deposits were not obvious by Alizarin Red staining after 14 d in the control group (x40). (B) Mineralized deposits were obvious by Alizarin Red staining after 14 d in the test group (x40). (C) ALP activity in the test groups rose at 3, 7, and 14 d. ICA indicates ICA-SF/PLCL drug-loaded nanofibrous membrane group.