Figure 6
From: Bark and wood tissues of American elm exhibit distinct responses to Dutch elm disease
Expression of JA-biosynthesis genes in infected and non-infected tissues of ‘Valley Forge’ and ‘Princeton’. (a–h) The relative normalized expression of genes encode linoleate 13S-lipoxygenase (13S-LOX), allene oxide synthase (AOS), allene oxide cyclase (AOC) and OPDA-reductase 3 (OPR3), respectively, in the mock-inoculated (0 hpi) and inoculated bark (a,c,e,g) and core (b,d,f,h) tissues of Valley Forge’ (VF) and ‘Princeton’ (P). The expression of each gene was normalized to that of three elm reference genes (EIF 5a, vacuolar ATP synthase and splicing factor 3B) and was calculated relative to the control sample (P-bark at 0 hpi). The results are the mean ± SE of three biological replicates. Values marked with an asterisk (*) are significantly greater (≥2 times) than the control sample and the corresponding time point in the other genotype (P < 0.05). (i) A schematic overview of the major steps and enzymes in the JA biosynthesis pathway.
