Figure 4 | Scientific Reports

Figure 4

From: Early involvement of cellular stress and inflammatory signals in the pathogenesis of tubulointerstitial kidney disease due to UMOD mutations

Figure 4

Characterization of infiltrating cells in kidneys of TgUmodC147W mice at 1 month of age. (a) Analysis by RT-qPCR of the expression of markers of infiltrating cells in male (upper panel) or female (bottom panel) TgUmodC147W mice relative to age- and sex-matched TgUmodwt mice (n = 5/group). Cd45 (Ptprc, common leukocyte marker), Cd68 (macrophage), Cd15 (Fut4, granulocyte) transcripts were highly expressed, while Cd5 (T cells) and Cd19 (B cells) were barely detectable (data not shown). Data are expressed as mean ± s.e.m. *P < 0.05; **P < 0.01; ***P < 0.001 (unpaired t-test). (b) Representative immunohistochemistry (IHC) staining for macrophage marker F4/80 in kidneys of TgUmodC147W and TgUmodwt mice. IHC analysis shows focal increase (arrowhead) of macrophage infiltrate in TgUmodC147W mice (scale bar 100 µm).

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