Figure 1 | Scientific Reports

Figure 1

From: Age-dependent motor dysfunction due to neuron-specific disruption of stress-activated protein kinase MKK7

Figure 1

Generation of neuron-specific MKK7 conditional knockout mice. (a) Immunoblot analysis of phospho-JNK in the mouse brain from embryonic to adult stages. Sor indicates sorbitol treatment as a positive control of JNK activation. β3-tublin (β3-tub) is the loading control. (b) Immunoblot analysis of phospho-JNK in adult mouse tissues. Extracts of tissues from WT mice were normalized by protein concentration. (c) Southern blotting analysis of Mkk7 gene deletion. HindIII-restricted genomic DNA was prepared from 3 week-old mice of the indicated genotypes. (d) Analysis of regions expressing Syn-Cre by β-gal staining. Brain and lumber spinal cord were prepared from 4 month-old LacZ reporter mice and Syn-Cre/LacZ double transgenic mice. Brain: Scale bar indicates 1 mm; Spinal cord: Scale bar indicates 300 μm. (e) Analysis of MAPKs activation in brain. Extracts of brain from 3 month-old MKK7 cKO and control mice were prepared and immunoblotted to detect MKK7, MKK4, JNK, ERK, p38 and c-Jun activities. GAPDH is the loading control. (f) Body weight of MKK7 cKO mice and control mice from 1–12 week-old. Mkk7 flox/flox , Mkk7 flox/+, Mkk7 flox/+ Syn-Cre and Mkk7 flox/flox Syn-Cre: n > 3.

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