Figure 2 | Scientific Reports

Figure 2

From: Proteomics/phosphoproteomics of left ventricular biopsies from patients with surgical coronary revascularization and pigs with coronary occlusion/reperfusion: remote ischemic preconditioning

Figure 2

Western blot analysis of phosphorylation/expression of STAT5 in human and of STAT3 in porcine left ventricular biopsies. (a) The phosphorylation of signal transducer and activator of transcription 5 (STAT5) at tyr694 tended to be increased at early reperfusion with remote ischemic preconditioning (RIPC; black symbols) compared to that with sham (white symbols) in human left ventricular (LV) biopsies lysed in Tris/sodium dodecyl sulfate (SDS) or radioimmunoprecipitation assay (RIPA) buffer. (b) The phosphorylation of signal transducer and activator of transcription 3 (STAT3) at tyr705 tended to be increased from baseline to early reperfusion after coronary occlusion with RIPC, and tended to be greater with RIPC than with sham in porcine LV biopsies. Immunoreactivities of the phosphorylated proteins were normalized to the respective total forms and compared by unpaired (between RIPC and sham) or by paired (between baseline and early reperfusion in pigs) Student’s t-tests. The blots were cropped to display the relevant bands, full-length blots and Ponceau-S stainings are presented in Supplemental Fig. S4.

Back to article page