Figure 5

Flow chart of sampling and proteome analysis of porcine left ventricular biopsies. Porcine left ventricular (LV) biopsies from pigs without (sham) or with remote ischemic preconditioning (RIPC) were taken at baseline and at early reperfusion and lysed in Tris/sodium dodecyl sulfate buffer (Tris/SDS). Proteome analysis was performed after phosphopeptide enrichment and in-solution digestion. The numbers of all detected phosphopeptides/proteins were displayed in line (a), those with ≥2-fold higher phosphorylation/expression in line (b), these with significant (p < 0.05) ≥2-fold higher phosphorylation/expression between groups (RIPC/sham) or between time points (baseline/early reperfusion), respectively, in line (c), and those exclusively detected in one group (RIPC/sham) or at one time point (baseline/early reperfusion) in line (d). The sum of lines (b), (c) and (d) was displayed in line (e). All detected phosphopeptides/proteins (line (a)) were subjected to false discovery rate (FDR)-based statistical analysis. All proteins detected at early reperfusion with a ≥2-fold higher phosphorylation/expression with RIPC than with sham (line (f)), were considered for an in-silico pathway analysis.