Figure 6

Vulcano plots of all detected phosphopeptides after phosphoproteome phosphopeptide enrichement of porcine left ventricular biopsies. Vulcano plots of −Log(10)p-value over -fold higher phosphorylation of all proteins between groups (remote ischemic preconditioning (RIPC)/sham) detected after phosphopeptide enrichment of porcine left ventricular biopsies taken (a) at baseline and (b) at early reperfusion. Vulcano plots of −Log(10)p-value over -fold higher phosphorylation of all proteins in the comparison between time points (baseline versus early reperfusion) (c) with remote ischemic preconditioning (RIPC) and (d) with sham. A −Log(10)p-value of ≥1.3 corresponds to a p-value of ≤0.05. The false discovery rate (FDR) significance cut-off curve indicates differences in protein phosphorylation between RIPC and sham at early reperfusion and between time points (baseline/early reperfusion) with RIPC and with sham, respectively. Grey squares: phosphopeptides without FDR-based statistical difference between RIPC and sham or between baseline and early reperfusion. Black squares: phosphopeptides with FDR-based statistical difference between RIPC and sham or between baseline and early reperfusion.