Figure 2
From: Double-stranded RNA induces chicken T-cell lymphoma apoptosis by TRIF and NF-κB
The activation of caspase pathway in MDCC-MSB1 cells cultured with poly (I:C). (A) The activities of caspases 3/7, 8, and 9 were measured in MDCC-MSB1 cells cultured with poly (I:C) (10 μg/ml) for 8, 16 and 24 h.The relative activity of the caspases was calculated as in Materials and Methods. (B) MDCC-MSB1 cells were cultured for 24 h with or without poly (I:C) (10 μg/ml), then stained with 5 μM JC-1 dye for 10 min at 37 °C to evaluate mitochondrial membrane potential integrity. Mitochondria with normal membrane potentialare shown in red, and mitochondria experiencing a loss of membrane potential are indicated in green. (C) MDCC-MSB1 cells were pre-treated with pan-caspase inhibitor Z-VAD-FMK, Necrostatin-1 or DMSO (used as control) before culture with or without poly (I:C) (10 μg/ml) for 24 hours. The percentage of cell viability is shown. (D) MDCC-MSB1 cells were pre-treated with the pan-caspase inhibitor Z-VAD-FMK, Necrostatin-1 or DMSO (used as control) before culture with or without poly (I:C) (10 μg/ml) for 24 hours. Results are expressed as a percentage of the apoptotic cells. (E) The caspase activity of MDCC-MSB1 cells, obtained as described in (A), was measured. The asterisk (*) or double asterisk (**) respectively indicates p < 0.05 or p < 0.01 in statistical difference from controls. The bars represent an average of multiple experiments.
