Table 1 Primers used for real-time PCR.
From: Double-stranded RNA induces chicken T-cell lymphoma apoptosis by TRIF and NF-κB
Target Gene | Sequence | Product size (bp) | Accession number |
|---|---|---|---|
GAPDH | F 5′-AGGGTGGTGCTAAGCGTGTTA-3′ | 78 | NM_204305.1 |
R 5′-TCTCATGGTTGACACCCATCA-3′ | |||
TRAF6 | F 5′-TCTGTTTGTCCACACGATGC-3′ | 145 | XM_421089.2 |
R 5′-TATCTCTGGCTTGGCTTCCA-3′ | |||
TLR3 | F 5′-GCACCTGTGAAAGCATTG-3′ | 99 | NM_001011691.3 |
R 5′-TAGGCGGGGTGTTACAAATG-3′ | |||
TRIF | F 5′-CACAGACCTTGCAATCCTCA-3′ | 134 | NM_001081506.1 |
R 5′-ATCACTGGTGCTCACTTCAC-3′ | |||
TANK | F 5′-GAGGAGTGGGCCAAGATGAG-3′ | 185 | NM_001277871.1 |
R 5′-GTCTCTGTGCTCCCGGTTAAA-3′ | |||
IKKα | F 5′-CTTTCATCTATGGCAACTCCTG-3′ | 244 | NM_001012904.1 |
R 5′-ATGTCCAAACCAAGACGTGAT-3′ | |||
IRF3/7 | F 5′-GAGCCTCCTCCCTCAACAGT-3′ | 118 | NM_205372.1 |
R 5′-AGGGACACAGGAAGGGAGTG-3′ | |||
NF-κB p65 | F 5′-GCCAGGTTGCCATCGTGTTCC-3′ | 181 | NM_205129.1 |
R 5′-CGCGTGCGTTTGCGCTTCTC-3′ | |||
IFN-β | F 5′-GCCCACACACTCCAAAACACTG-3′ | 151 | NM_001024836.1 |
R 5′-TTGATGCTGAGGTGAGCGTTG-3′ | |||
RIP1 | F 5‘-AGTGCTCCAAAAAGTCCCAGTACC-3′ | 211 | XM_015275820.1 |
R 5′-GGTCTCTTCTTTGGTCAGCCG-3′ | |||
TNF-α | F 5′-TGTGTATGTGCAGCAACCCGTAGT-3′ | 229 | NM_204267.1 |
R 5′-GGCATTGCAATTTGGACAGAAGT-3′ | |||
Bak | F 5′-ATGGATGCCTGTCTGTCCTGTTC-3′ | 106 | NM_001030920.1 |
R 5′-GCAGAGCAGTCCAAAGACACTGA-3′ | |||
p53 | F 5′-GTCCCATCCACGGAGGATTATG-3′ | 92 | NM_205264.1 |
R 5′-GAGTAAGTGCAGGTGACCGATT-3′ | |||
Fas | F 5′-CTTCTCGGTGTGAACATTGCG-3′ | 219 | NM_001199487.1 |
R 5′-GCTGGTGGGTCAGGTCAACATC-3′ | |||
Bcl-2 | F 5′-GATGACCGAGTACCTGAACC-3′ | 114 | NM_205339.2 |
R 5′-CAGGAGAAATCGAACAAAGGC-3′ | |||
Bcl-xl | F 5′-TCCTCATCGCCATGCTCAT-3′ | 69 | NM_001025304.1 |
R 5′-CCTTGGTCTGGAAGCAGAAGA-3′ |
