Figure 3
From: Circadian Clock Model Supports Molecular Link Between PER3 and Human Anxiety
Validation of model fitness using knockout conditions. Concentration data for (A–H) obtained from a representative example parameter set (See Table S2 for set). (A) After PER1 knockout, BMAL1 mRNA has a shorter period, as observed experimentally (p < 0.001)55. (B) After PER2 knockout, BMAL1 mRNA has a shorter period relative to WT conditions (p < 0.001)55. (C) After PER3 knockout, BMAL1 mRNA has a shorter period (p < 0.001)61. (D) After CRY1 knockout, BMAL1 mRNA levels display a shorter period (p < 0.001)61. (E) After CRY2 knockout, BMAL1 mRNA displays an increase in period length relative to WT conditions (p < 0.001)61. (F) After a double knockout of CRY1 and CRY2, BMAL1 mRNA levels are arrhythmic 61. (G) After NPAS2 knockout, BMAL1 mRNA levels are unaffected 61. Due to the minimal effect of this knockout, the concentration plots overlap. (H) After BMAL1 knockout, PER2 mRNA levels decrease and become arrhythmic. (I) Bar graph of normalized period levels after knockouts. Error bars represent two standard errors from the mean.
