Figure 3

High fat diet-fed mice exhibit accelerated spontaneous OA. (A) Representative histological sections of Safranin-O/Fast Green stained knee joints from lean diet (LD) and high fat diet (HFD)-fed mice at 9 months and 12 months of age. Magnifications: 10x and 20x. (B) Severity of OA pathogenesis was determined by OARSI histopathology grading of mouse femoral condyles and tibial plateaus and number of chondrocytes per area were counted. Data are expressed as mean ± SD. Data were analysed by Two-way ANOVA followed by Holm-Sidak multiple comparison tests. */⁺p < 0.05; n = 10. ⁺p < 0.05 LD vs HFD at 9 months of age LD vs HFD at 12 months of age. *p < 0.05 HFD at 9 months of age vs HFD at 12 months of age and LD at 9 months of age vs LD at 12 months of age (n = 10).