Figure 1 | Scientific Reports

Figure 1

From: The Transcription Factor DAF-16 is Essential for Increased Longevity in C. elegans Exposed to Bifidobacterium longum BB68

Figure 1

Bifidobacterium longum BB68 extends the lifespan of C. elegans by regulating TIR-1 – JNK-1 – DAF-16 signaling. (A) Feeding worms BB68 increased the lifespan of the N2 and daf-2 strains, but not the daf-16 strain. (B) Feeding DAF-16::GFP worms BB68 for 24 h activated DAF-16 (the arrows indicate increased nuclear accumulation of DAF-16), and lower levels of activated DAF-16 were detected in the presence of mutated jnk-1 or tir-1. (C) Western blotting showed significant nuclear accumulation of DAF-16 protein in nematodes fed BB68 for 24 h. Lamin B1 was used as a nuclear reference, and GAPDH was used as a cytosolic reference. (D) An increase in SOD-3 gene expression level was observed in nematodes fed BB68 for 24 h. **Indicates a significant difference (Student’s T-test, p < 0.01). The test was conducted in triplicate. (E) The presence of mutant jnk-1 and tir-1 completely eliminated the lifespan extension induced by BB68. Scale bar = 50 μm.

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