Figure 2

Feeding N2 worms BB68 for 24 h activated JNK-1 via phosphorylation. The phospho-JNK/SAPK (Thr183/Tyr185) antibody and Cy3-labeled goat anti-mouse IgG (H + L) were used as primary and secondary antibodies, respectively. The dashed circle indicates phosphorylated JNK-1 in the nerve ring, but no activated JNK-1 was detected in the presence of mutant tir-1.