Figure 1

Lytic cell debris generated following cytolytic DNA expression can activate DCs. (A) HEK293T cells were transfected for 48 hours with 200 ng of DNA or treated for 24 hours with 2 μM doxorubicin prior to measuring LDH release into the culture supernatants. Data show the mean (n = 6) + SEM of specific LDH release from two independent experiments. (B) Magnetically enriched CD11c⁺ cells from naïve C57BL//6 mice (n = 3) were exposed to transfected HEK293T cells or heat-induced necrotic cells in the presence of 0.1 μg/ml SIINFEKL peptide for 8 hours prior to flow cytometric analysis as described in the methods. Representative histogram plots show CD80 and CD86 expression on gated CD11c⁺ I-Ab⁺ (total), CD8α⁺ CD11c⁺ I-Ab⁺ and CD8α⁻ CD11c⁺ I-Ab⁺ cells. Similar results were observed in two independent experiments. (C) Mean (n = 3) + SEM for the data described in B. A paired t-test was used to determine the statistical significance of the data. (D) Representative histogram plots showing SIINFEKL-H2-K^b (25-D1.16) expression on gated DC populations for the experiment described in B.