Figure 1 | Scientific Reports

Figure 1

From: GSK3β and ERK regulate the expression of 78 kDa SG2NA and ectopic modulation of its level affects phases of cell cycle

Figure 1

NIH3T3 has only 78 kDa isoform enriched in cytosol and microsomes. (A) NIH3T3 cells were harvested and 75 μg of protein lysates were loaded on SDS-polyacrylamide gel and blotted with SG2NA antibody. Protein was extracted from transiently transfected HEK293T cells harbouring various isoforms of SG2NA i.e., 35 kDa, 52 kDa, 78 kDa and 87 kDa were loaded to act as markers. (B) NIH3T3 cells were fractionated and each organelle rich fraction was loaded and blotted with SG2NA antibody, authenticity of the fractions were confirmed by probing with organelle specific markers. Full length blots for panel (B) are shown in Supplementary Figure S1.

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