Figure 4 | Scientific Reports

Figure 4

From: GSK3β and ERK regulate the expression of 78 kDa SG2NA and ectopic modulation of its level affects phases of cell cycle

Figure 4

Phosphorylation of SG2NA is oscillated during the course of cell cycle and is organelle specific. (A) NIH3T3 cells were synchronised by double thymidine block and harvested at different phases as G1, S, G2 at 0 hr, 3 hrs and 8 hrs after release from thymidine block. Then cell lysates were immunoprecipitated with SG2NA antibody followed by probing using phosphoserine, phosphothreonine, SG2NA and β-actin antibodies. (B) Graph representing relative level of proteins with respect to β-actin as a control. (C) NIH3T3 cells were fractionated and organelle enriched fractions were immunoprecipited with SG2NA antibody and then probed with phosphoserine, phosphothreonine and SG2NA antibodies. (D) Graph representing level of proteins in various organelle riched fractions. Error bars are the standard deviation of 2 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. Full length blots for panel (A) and (C) are shown in Supplementary Figure S1.

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