Figure 6

SG2NA also regulates the level of pERK and pGSK3β. (A) NIH3T3 and SG2NA downregulated NIH3T3 cells were harvested and loaded on SDS-polyacrylamide gel, transferred then probed with various antibodies like SG2NA, pERK, total ERK, pGSK3β and β-actin. (B) Graph representing relative expression of proteins with β-actin as loading control is shown. Error bars are the standard deviation of 2 independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. Full length blots for panel (A) are shown in Supplementary Figure S1.