Figure 2 | Scientific Reports

Figure 2

From: Drosophila protease ClpXP specifically degrades DmLRPPRC1 controlling mitochondrial mRNA and translation

Figure 2

Protein levels of DmLRPPRC1 and DmSLIRP1 in ClpP-depleted Schneider S2 cells. Schneider S2 cells with no plasmid (control) or carrying pMt/invGFP/Hy (GFP RNAi) or pMt/invClpP/Hy (ClpP RNAi) or pMt/invLon/Hy (Lon RNAi) were cultured for 5 d in the presence or absence of 0.1 mM CuSO4. (A) Immunoblot analysis was carried out as described in the legend to Fig. 1A. (B) The relative protein levels of ratios ClpP, Lon, DmLRPPRC1 or DmSLIRP1. The ratios were normalized as described in Fig. 1B. Data represent the mean ± SD of four independent experiments. *P < 0.05; ***P < 0.001 versus control cells without induction (Student’s t-test). (C) The relative ratio of DmLRPPRC1 and DmSLIRP1 mRNA. The ratio was determined as described in Fig. 1D. Data represent the mean ± SD of three independent experiments. Full blots are presented in Supplementary Fig. S9.

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