Figure 4
Dynamics of DmLRPPRC1 and DmSLIRP1 proteins during mitochondrial transcript depletion in ClpP or Lon knockdown Schneider S2 cells. (A) Schneider S2 cells with no plasmid (control) or carrying pMt/invGFP/Hy (GFP RNAi) or pMt/invClpP/Hy (ClpP RNAi) or pMt/invLon/Hy (Lon RNAi) were cultured for 2 d in the presence or absence of 200 ng∕mL EtBr. Upper panel, immunoblot analysis was carried out as described in the legend to Fig. 1A. Lower panel, reduction ratio of DmLRPPRC1 and DmSLIRP1 protein with or without EtBr treatment. Relative ratio of each cell lines was determined as described in Fig. 1B and normalized by the ratios of each cells without EtBr treatment. The results represent the mean ± SD of three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001 versus each untreated cells. (B) Schneider S2 cells carrying no plasmid (control) and or pMt/invClpP/Hy (ClpP RNAi) were cultured for 8 d in the absence (non-treated) or presence of dsRNA of DmLRPPRC1 (dsLRPPRC1), DmSLIRP1 (dsSLIRP1) or GFP (dsGFP) as control. Upper panel, immunoblot analysis was carried out as described in the legend to Fig. 1A. Lower panel, the relative DmLRPPRC1 or DmSLIRP1 protein ratio with or without dsRNA treatment. Relative ratio of each cell lines was determined as described in Fig. 1B and normalized by the ratios of each cells without dsRNA treatment. **P < 0.01; ***P < 0.001 versus each untreated cells. Full blots are presented in Supplementary Fig. S9.
