Figure 3
Silencing synaptic transmission of local interneurons type 1 (LN1). (A) Synaptic transmission of LN1 was silenced by expressing shi ts under the control of the enhancer trap line NP1227-GAL4 while calcium signals were monitored in OSNs (left: Orco) and PNs (right: GH146, see scheme part (ii) in Fig. 1 for details). False color coded mean activity of 15 glomeruli for the three experimental phases during the presentation of ISO (upper panels) and BEA (lower panels). (B) Comparison of the odor-evoked maximal fluorescence distributions between the parental control (UAS-shi) and the experimental line (LN1 > shits) reveals no significant differences between the permissive and non-permissive temperatures, neither for the OSN nor for the PN activity (ANOVA, p > 0.05). (C) The odor evoked population vector activity (shown in A) was used to calculate Euclidean distances between both odor representations separately in every experimental phase. The temperature increase to 31 °C led to a drastic decrease in the Euclidean distances (red) in OSN (left) as well as in PN activity (right). At the permissive temperature during the recovery phase, the Euclidean distances increased again but did not completely recover. However, we observed the same effect for the parental control (UAS-shi OSNs, N = 9; UAS-shi PNs, N = 10).
