Figure 5

Silencing synaptic transmission of LN1 or LN2 has no influence on the spatial glomerular activity. (A) Principal component analysis of odor-evoked calcium responses of flies with silenced LN1-output for the OSN (left) and the PN level (right). Trajectory plots of the first three principal components (PC1, 2 and 3) show a clear separation of ISO and BEA in all experimental phases at both processing levels. The variation explained by the individual PCs is given at the axes. Black arrows mark the baseline activity before odor onset. During temperature increase (silencing LN1-output) the trajectories are strongly collapsed at the OSN level. The factor loadings (weights) of the single glomeruli were used to order them with respect to their contribution to the variability of PC1 for the OSN activity (most left) and the PN activity (most right). The false color coded matrix including the averaged mean fluorescence change is shown for both processing levels with respect to this ordering. The spatial activity patterns at both processing levels (OSNs and PN) were not affected by the temperature shift. However, the calcium signal strength decreased strongly during the temperature increase and recovered during the permissive temperature in the recovery phase (22 °C (post)). (B) Same representation as in A for flies with silenced LN2-output.