Figure 3

NMDA receptor antagonist, D-AP5, blocked the formation of LTP in the Dl-Dm pathways of both the contralateral and ipsilateral side. (a) Effect of D-AP5 on LTP induction in the contralateral side with Dm induction (group data). (b) Effect of D-AP5 on LTP induction in the ipsilateral side with Dm induction (group data). Here, 30 µM of D-AP5 (solid line) was applied after 10 min of baseline recording. The first HFS (arrow) was delivered 15 min after the start of drug perfusion (point 2 compared to point 1, contralateral: 87.57 ± 3.97%, n = 9, p = 0.0576; ipsilateral: 104.3 ± 9.93%, n = 9, p = 0.9529). The second HFS (arrow) was delivered 30 min after the washout of D-AP5. LTP was not induced under the effect of D-AP5 (point 3 compared to point 1, contralateral: 100.8 ± 7.71%, p = 0.9977; ipsilateral: 100.5 ± 7.59%, p = 0.999), but the second HFS was able to induce LTP after D-AP5 washout, which lasted at least 1 hour (point 4 compared to point 1, contralateral: 194.5 ± 28.97%, p = 0.0323; ipsilateral: 168.2 ± 11.78%, p = 0.0012). Values for each side were tested by using one-way ANOVA. Post hoc analyses were conducted using Dunnett’s test. Each point represents the mean ± SEM of the PS amplitude. n = 9.