Figure 1 | Scientific Reports

Figure 1

From: CD24 induces changes to the surface receptors of B cell microvesicles with variable effects on their RNA and protein cargo

Figure 1

The quantification and morphology of MVs released from B cells with and without CD24 stimulation. (A) Representative nanoparticle tracking (NTA) plots of particle sizes and concentrations in supernatants from cells stimulated with either isotype (right) or anti-CD24 (left) Ab after 1 h. Grey lines show ± 1 standard deviation of the mean in measurements. n = 3 biological replicates with 5 technical replicates each. (B) The mean size (left) and concentration (right) ± SEM of particles from supernatants analyzed by NTA, n = 3. Statistical significance was assessed using Students’ paired t-test, #p = 0.08. (C) Mean % positive (left) and relative mean fluorescent intensity (MFI; right) of Annexin V-FITC beads used to capture CD24-bearing MVs released from isotype or anti-CD24 stimulated cells for the indicated times, n = 3. Statistics assessed by a two-tailed Student’s paired t-test. **p < 0.01. (D) Representative transmission electron microscopy (TEM) images of Vn96-isolated EVs from cells stimulated with either isotype or anti-CD24 for 1 h. Scale bar = 100 nm.

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