Figure 3
Proteomics analysis suggests that CD24 stimulation causes enrichment of proteins from specific functional categories into MVs. Gene Ontology (GO) enrichment analysis was performed on proteins common to MVs from all 6 isotype and anti-CD24 samples in comparison to the proteins enriched in MVs after 1 h anti-CD24 stimulation (Supplemental File 1). Venn diagrams (left panels) show GO terms associated with all MVs or those associated with the proteins enriched in MVs after anti-CD24 stimulation. The numbers indicate the number of unique GO terms associated with the respective protein lists. Enriched GO terms were visualized by Revigo70 (right panels). (A) CD24-enriched biological process (BP): Cellular amide metabolism (yellow), macromolecular complex assembly (green) and protein localization to nuclear body (blue). (B) CD24-enriched molecular functions (MF): Damaged DNA binding (green), transferase activity (purple) and threonine aldolase activity (blue). (C) CD24-enriched cellular component annotations were primarily grouped as chaperonin-containing T-complex (red). A.A. = amino acid, Synth = synthesis, Carb = carboxylic, Reg = regulation, Pos. = positive, Leng. = length, org = organization, Maint = maintenance, Loc = localization, Est = Establishment, Spm = sperm, Z.P = zona pellucida.
