Figure 5

CD24 stimulation induces the formation of a unique B cell MV surface phenotype that does not reflect the cells from which they are released. Cells (A,B) and MVs (C,D) were analyzed for their expression of the indicated cell surface markers. Data are shown as mean ± SEM at 1 h or 2 h stimulation with either isotype or anti-CD24 Ab. n = 3–4. Cells or CD24-bearing MVs bound to beads were assessed for the percentage of (A) cells or (C) MVs positive for the individual markers, and (B/D) their relative mean fluorescent intensities (MFI). Significant differences were assessed by two-way ANOVA followed by Tukey post-hoc, if significant. Main effect of stimulation: ^§P < 0.05, ^§§P < 0.01. Significant changes compared to time-matched control: ^#P < 0.1, *P < 0.05, **P < 0.01. Non-statistically significant changes have no indicators.