Figure 2

HO-1 mRNA expression is differently regulated in response to oxidative stress before and after cell differentiation. RT-PCR analysis of HO-1(a), GCLM (b) and GCLC (c), Nrf2 (d) and Bach1 (e) in undifferentiated and 4d-ATRA differentiated cells treated for 6 h with 500 μM H2O2 or 50 µM tBHQ - positive control of HO-1 induction - as indicated. Statistical analysis: n = 3 *p < 0.05 vs control; **p < 0.01 vs control; #p < 0.01 vs tBHQ.