Figure 5
Evaluation of T2 transgenic lines against sheath blight disease along with non-transformed control through In vitro and In vivo plant bioassay. (a) Mycellial agar disc bioassay showing reduced infection cushion formation in T2 transgenic lines (CN5-2-1, N4-3-2, C8-9-1) than in the wild-type (WT). Experiments replicated three times. (b) Representative images of reduced lesion formation in transgenic leaves (CN5-2-1, N4-3-2, and C8-9-1) relative to WT in mycellial agar disc bioassay. (c) Bar diagram showing percentage of affected area after 72 hrs post infection in transgenic leaf samples than wild type (WT) in RS -toxin bioassay. (d) Images showing less affected area in transgenic leaves (CN5-2-1, C8-9-1, and N4-3-2) compared to WT control in the toxin bioassay. (e) Image showing reduced sheath blight symptoms development in rice tillers in T2 transgenic plants, than that in WT control after 21 dpi (Days post infection). (f) Percentage of infected area on tillers on T2 transgenic (CN5-2-1, C8-9-1, and N4-3-2) and WT plants after 21 dpi. (g) Images showing sheath blight symptoms on leaf blade of transgenic (CN5-2-1, C8-9-1, and N4-3-2) and non-transformed control. (h) Lesion size on leaf blade of WT and transgenic lines after 21 dpi. (i) Lesion size on leaf sheath of both WT and transgenic lines (CN5-2-1, C8-9-1, and N4-3-2) after 21 dpi. Values are presented as mean of 10 replicas ± SE. (j) Representative images showing reduction in symptoms development on rice sheath of transgenic lines as compared to WT after 21 dpi.
