Figure 3
Scanning electron micrographs of derivatives of MinC expression in E. coli BL21(DE3) or MinC-KO. (A) E. coli BL21(DE3) (WT); (B) EcMinC-Aahelix3-BL21(DE3); (C) EcMinCCTD-BL21(DE3); (D) EcMinCCTD-Aahelix3-BL21(DE3); (E) BW25113; (F) MinC-KO; (G) EcMinC-MinC-KO; (H) EcMinC-Aahelix3-MinC-KO. Cells of EcMinCCTD-Aahelix3 exhibited much longer cell morphology than WT (A~D) and complementation of MinC-helix3 to MinC-KO strains could not help cells regain the ability of cell division regulation (E~H), verifying that the Aahelix3 influenced the normal cell division in cells. Scale bars represent 5 μm.
