Figure 2

Association between bpifcl mRNA and kiss2 mRNA expression. bpifcl mRNA expression in zebrafish brain during development (A) and between 6 months old males and females (B). kiss2 mRNA expression between 6 months old males and females (C). Effect of 11-KT exposure on bpifcl (D) and kiss2 (E) mRNA expression in 6 months old male zebrafish brain. Effect of E2 exposure on bpifcl (F) and kiss2 (G) mRNA expression in 6 months old male zebrafish brain. Expression levels of 45 days post-fertilisation (dpf) (A), males (B,C) and 0 μg/L exposure (D–G) were defined as 1.0. All data are presented as the mean ± standard error of the mean and were analysed by a Student’s t-test for sex differences and one-way analysis of variance and Tukey’s post-hoc test for multiple comparisons for the others. **p < 0.01.