Figure 5
From: Absolute measurement of gene transcripts with Selfie-digital PCR
Absolute measurement of RNA interference by digital Selfie-dPCR. To obtain an absolute value of gene silencing by RNA interference, lentiviral-mediated overexpression of Gsk3β was silenced by co-transduction of a short hairpin Gsk3β for RNA interference (shGsk3β) in an N2a neuroblastoma cell line. (A) Schematic diagram showing the positions of the sequence targeted by shGsk3β (in green) and the Gsk3β-81 amplicon. (B) Quantification of Gsk3β transcripts per genome after lentiviral-mediated Gsk3β transgene overexpression and silencing. (C) Number of proviruses integrated per genome, as assessed by the number of WPRE copies.
