Figure 2 | Scientific Reports

Figure 2

From: Multimerization is required for antigen binding activity of an engineered IgM/IgG chimeric antibody recognizing a skin-related antigen

Figure 2

Production and characterization of various IgM/IgG hybrid molecules. (a) Non-reducing Western blotting analysis of culture supernatant of HEK293T transfected with cDNA for the indicated IgM/IgG molecules using anti-mouse kappa chain specific-HRP. (b) Analysis of Protein G binding to various IgM/IgG hybrid molecules. Western blotting was performed under non-reducing condition using anti-mouse kappa chain specific-HRP. (c and d) Analysis of binding of anti-mouse IgG (c) and anti-mouse IgM (d) to the heavy chains of various IgM/IgG hybrid molecules. ALP-conjugated anti-mouse IgG or IgM was used as secondary antibody. Cropped blots are shown and full length blots are shown in Supplementary Fig. S1.

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