Figure 7 | Scientific Reports

Figure 7

From: Multimerization is required for antigen binding activity of an engineered IgM/IgG chimeric antibody recognizing a skin-related antigen

Figure 7

Gel filtration and immunofluorescence of normal human skin using (ii) VH-Cγ1-Hinge-Cγ2-Cγ3 with 309 C and μTP mutations. (a) Presentation of gel filtration chromatographs for (ii) VH-Cγ1-Hinge-Cγ2-Cγ3 with 309 C and μTP mutations. Fractions corresponding to the indicated peaks were collected and used for immunofluorescence of normal human skin. The elution position of monomer is indicated. (b,c) Immunofluorescence of normal human skin using the indicated gel filtration fractions as primary antibodies.

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