Table 1 Summary of properties of recombinant IgM and IgM/IgG molecules.

From: Multimerization is required for antigen binding activity of an engineered IgM/IgG chimeric antibody recognizing a skin-related antigen

 

(i) VH -Cµ1 -Cµ2 -Cµ3 -Cµ4

(ii) VH -Cγ1 -Hinge -Cγ2 -Cγ3

(iii) VH -Cµ1 -Hinge -Cγ2 -Cγ3

(iv) VH -Cµ1 -Cµ2 -Cγ3

(v) VH -Cµ1 -Cµ2 -Cγ2 -Cγ3

(vi) VH -Cµ1 -Cµ2 -Hinge -Cγ2 -Cγ3

Protein G binding

−

+

+

−

+

+

IgG-HC binding

−

+

+

+

+

+

IgM-HC binding

+

−

−

+

+

+

Skin IF

+

−(+)*

−(+)*

+

−(+)*

+(+)*

EE WB

+

−

−

+

−

+

  1. HC, heavy chain, IF, Immunofluorescence, EE, epidermal extract, WB, Western blotting, H, Hinge, *(With single or double 309 C and µTP mutations).
Back to article page