Figure 6
Foot process and filtration slit in WT and CD2AP deficient mice by HIM. HIM images of foot processes and SDs in WT (A) and 3 week-old Cd2ap-KO (B–D) kidney. Image of WT (A) shows interdigitated foot processes forming evenly spaced filtration silts. The slit pores (black arrows) line up and form a ladder-like pattern in the middle of the filtration slit and between adjacent foot processes. Image of Cd2ap-KO kidney (B) shows the disorganized and irregular orientation/alignment of primary process and foot processes. Foot process branching appears more random, and foot processes show flattened and widened (B). The “gaps” between disrupted SDs in some areas are detectable (white arrows, C,D). The junction between foot processes appears closed in some areas (white arrowhead, C). Scale bars, 100 nm (A,B); 50 nm (C,D). MP, major process; FF, foot process.
