Figure 2
Behaviors of amniogenic somatopleural cells contributing to intraembryonic tissues. (a–c) Representative images of DiI-labeled somatopleural cell distribution to intraembryonic tissues including the thoracic wall, pharyngeal arch and cardiac outflow tract (yellow arrowheads). (d–i) Two-color dye labeling reveals a trajectory of somatopleural cells toward the pharyngeal arch through the pericardial fold. Both CFDA/DiO (green) and DiI (red) labels likely draw trajectories dorsoventrally on the amnion, whereas only DiI labels direct towards the ventral tip of the first pharyngeal arch on the forming thoracic wall (i). (j–l) Two-color dye labeling reveals that somatopleural cells and cells in the lateral edge of the head fold are likely to distribute differently with a boundary around the first pharyngeal arch. Dye-injected sites (red arrowheads in a,d,j) and later distributions (b,e in bright field; c,f–h,k,l in dark field) are shown. am, amnion; la, left atrium; lb, limb bud; opv, optic vesicle; otv, otic vesicle; pa1, first pharyngeal arch; pa2, second pharyngeal arch; th, thoracic wall; *, edge of the closing pericardial fold.
