Figure 3
Sequential imaging of the dye-labeled cells in the amniogenic somatopleure forming the amniotic folds. (a) Dorsal view of sequential imaging of every 3 hours for 21 hours. DiI is injected in the amniogenic somatopleure in the right side of the head (red arrowhead). After 6 to 9 hours, the dye-labeled cells (yellow arrowheads) segregate into two groups; one is the mesodermal layer (black arrows) and the other is the amniotic membrane with the ectodermal epithelium (white arrows). (b) Schematic depiction is superimposed on each image. (c–g) Right lateral view (c) and immunostaining for the epithelial cell marker cytokeratin on transverse sections (d–i) of the embryo shown in (a). Boxes in (d,e) are magnified in (f,g) and (h,i) respectively. am, amnion; ht, heart tube; nt, neural tube; oft, outflow tract; opv, optic vesicle; v, ventricle. Scale bars, 300 µm for (d) to (i).
