Table 1 Comparison of the recombination efficiencies of different orthologous exonuclease-recombinase pairs for linear dsDNA.
From: Recombineering using RecET in Corynebacterium glutamicum ATCC14067 via a self-excisable cassette
Vector | Kmr per 109 viable cells | ||
|---|---|---|---|
+dsDNA | −dsDNA | +plasmid | |
pEC-XC99E | 0 ± 0 | 0 | 3.1 + 0.21 × 103 |
pEC-bet/exo | 0 ± 0 | 0 | 3.8 ± 0.14 × 103 |
pEC-orf47/orf48 | 13 ± 2 | 0 | 3.6 ± 0.14 × 103 |
pEC-orfB/orfC | 59 ± 3 | 0 | 3.7 ± 0.14 × 103 |
pEC-recE/recT | 66 ± 5 | 0 | 3.8 ± 0.14 × 103 |
