Figure 3

Effect of Nej1° and Dnl4° on homologous recombination. (a) Schematic of the transforming donor DNA fragment targeting the Ura3 locus. Dashed boxes indicate homologous regions. (b), (c) Homologous recombination rates (5-FOA resistant (inserted)/zeocin resistant (transformants)). Fragments were prepared either by PCR or by restriction digestion of plasmid (Cut).