Figure 7 | Scientific Reports

Figure 7

From: Development of a comprehensive set of tools for genome engineering in a cold- and thermo-tolerant Kluyveromyces marxianus yeast strain

Figure 7

In vivo assembly and integration of three DNA fragments with 50 bp overlaps. (a) Schematic of the three transforming donor DNA fragments replacing the Ura3 locus along with the CRISPR-Cas9 plasmid targeting two sites (indicated by lightning bolts) flanking the Ura3 CDS. Dashed boxes indicate homologous regions. Bottom panel shows (b) Homologous recombination rate ((5-FOA resistant * PCR check)/ transformants (G418 resistant) * 100).

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