Table 1 Offspring body/organ weights, renal morphology and renal RAS mRNA expression at postnatal day 21.

From: Prenatal hypoxia leads to hypertension, renal renin-angiotensin system activation and exacerbates salt-induced pathology in a sex-specific manner

 

Control

Hypoxia

Two-way ANOVA

Male

Female

Male

Female

Treatment

Sex

Interaction

Body and organ weights

Bw (g)

11.86 ± 0.3

11.20 ± 0.3

10.95 ± 0.3

10.39 ± 0.4

P = 0.01

n.s.

n.s.

Brain (mg)

361 ± 9

360 ± 10

357 ± 17

379 ± 9

n.s.

n.s.

n.s.

Brain:bw

30.7 ± 1.4

32.5 ± 1.1

32.4 ± 1.8

37.7 ± 2.2

P = 0.04

P = 0.03

n.s.

Heart (mg)

75.2 ± 2.8

72.2 ± 1.6

72.4 ± 2.7

70.8 ± 3.3

n.s.

n.s.

n.s.

Heart:bw

6.27 ± 0.1

6.42 ± 0.1

6.5 ± 0.2

6.6 ± 0.1

n.s.

n.s.

n.s.

Kidney (mg)

83.1 ± 2.8

78.6 ± 2.8

75.9 ± 2.2

73.2 ± 3.4

0.03

n.s.

n.s.

Kidney:bw

6.94 ± 0.2

6.96 ± 0.1

6.81 ± 0.1

6.86 ± 0.1

n.s.

n.s.

n.s.

Renal morphology

Glomerular number

12888 ± 515

9854 ± 432

9782 ± 517**

9753 ± 440

P = 0.002

P = 0.004

P = 0.004

Glomerular area (um 2)

1964 ± 130

2140 ± 185

1797 ± 72

1778 ± 128

n.s.

n.s.

n.s.

Renal RAS mRNA expression

Renin

1.22 ± 0.27

1.45 ± 0.25

2.17 ± 0.43*

1.14 ± 0.17

n.s.

n.s.

P = 0.03

At 1a R

1.02 ± 0.07

1.13 ± 0.09

1.16 ± 0.11

1.02 ± 0.09

n.s.

n.s.

n.s.

Ace

1.08 ± 0.16

1.16 ± 0.19

1.37 ± 0.17

0.88 ± 0.08

n.s.

n.s.

n.s.

  1. Values are mean ± SEM. Organ/body weight (bw): N = 2 offspring from 11 litters/group. Glomerular number/area: N = 8–9/group. mRNA expression: N = 9–12/sex/litter. Effect of treatment, sex or interaction (treatment x sex) was evaluated by two-way ANOVA. *P < 0.05, **P < 0.001 comparing male control/hypoxia offspring with a Sidak post hoc. Not significant (n.s.).
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