Table 2 Offspring body and organ weights and renal parameters at 12 months of age.

From: Prenatal hypoxia leads to hypertension, renal renin-angiotensin system activation and exacerbates salt-induced pathology in a sex-specific manner

 

Normal Salt

High Salt

Two-way ANOVA

Control

Hypoxia

Control

Hypoxia

Treatment

Diet

Inter-action

MALES

  Body and organ weights

  Bw (g)

55 ± 2

55 ± 2

58 ± 3

57 ± 2

n.s.

n.s.

n.s.

  Kidney (mg)

724 ± 35

715 ± 19

841 ± 75

912 ± 26

n.s.

P = 0.002

n.s.

  Kidney:bw

13.2 ± 0.6

12.7 ± 0.4

14.6 ± 1.3

16.2 ± 0.7

n.s.

P = 0.02

n.s.

  Heart (mg)

253 ± 6

245 ± 5

273 ± 14

268 ± 7

n.s.

P = 0.007

n.s.

  Heart:bw

4.5 ± 0.1

4.5 ± 0.1

4.7 ± 0.1

4.8 ± 0.2

n.s.

n.s.

n.s.

  Renal parameters

  Urine output (ml/24 h)

1.73 ± 0.3

1.35 ± 0.3

2.01 ± 0.4

2.19 ± 0.5

n.s.

n.s.

n.s.

  Water consumption (ml/bw/24 h)

0.11 ± 0.008

0.14 ± 0.03

0.26 ± 0.05

0.19 ± 0.01

n.s.

P = 0.0003

n.s.

  Albumin excretion (µg/24 h)

9.7 ± 2.1

22.4 ± 3.8

8.2 ± 2.8

27.0 ± 13.0

P = 0.04

n.s.

n.s.

  UNaV (µmol/24 h)

133 ± 24

99 ± 27

400 ± 70

513 ± 52

n.s.

P < 0.0001

n.s.

  UClV (µmol/24 h)

154 ± 57

130 ± 89

374 ± 143

513 ± 145*

n.s.

P < 0.0001

P = 0.04

  UKV (µmol/24 h)

219 ± 23

210 ± 41

217 ± 37

228 ± 39

n.s.

n.s.

n.s.

  Plasma cystatin C (ng/mL)

768 ± 103

880 ± 109

645 ± 46

600 ± 30

n.s.

P = 0.02

n.s.

FEMALES

  Body and organ weights

  Bw (g)

53 ± 2

50.8 ± 4

54 ± 3

49 ± 3

n.s.

n.s.

n.s.

  Kidney (mg)

493 ± 10

481 ± 22

543 ± 37

582 ± 44

n.s.

P = 0.02

n.s.

  Kidney:bw

9.7 ± 0.7

9.1 ± 0.4

9.0 ± 0.4

10.4 ± 1.0

n.s.

n.s.

n.s.

  Heart (mg)

194 ± 13

189 ± 8

215 ± 13

220 ± 13

n.s.

P = 0.009

n.s.

  Heart:bw

3.8 ± 0.4

3.6 ± 0.2

3.6 ± 0.1

3.9 ± 0.3

n.s.

n.s.

n.s.

Renal parameters

Urine output (ml/24 h)

1.50 ± 0.3

1.57 ± 0.3

2.13 ± 0.3

3.02 ± 0.7

n.s.

n.s.

n.s.

Water consumption (ml/bw/24 h)

0.07 ± 0.005

0.074 ± 0.02

0.26 ± 0.05

0.18 ± 0.07

n.s.

P = 0.01

n.s.

Albumin excretion (µg/24 h)

51.5 ± 15.3

57.4 ± 18.0

39.6 ± 16.5

11.8 ± 5.1

n.s.

n.s.

n.s.

UNaV (µmol/24 h)

111 ± 21

76 ± 12

534 ± 101

479 ± 98

n.s.

P < 0.0001

n.s.

UClV (µmol/24 h)

158 ± 27

130 ± 21

546 ± 95

489 ± 92

n.s.

P < 0.0001

n.s.

UKV (µmol/24 h)

186 ± 26

170 ± 25

162 ± 24

120 ± 18

n.s.

n.s.

n.s.

Plasma cystatin C (ng/mL)

1246 ± 270

1166 ± 307

785 ± 58

866 ± 78

n.s.

P = 0.07

n.s.

  1. Values are mean ± SEM. Body weight (bw). Body/organ weights: N = 1–2 offspring from 11 litters/group. Renal parameters: male, N = 6–10/group; female, N = 5–10/group. Effect of treatment, diet or interaction (treatment x diet) was evaluated by two-way ANOVA. *Sidak post hoc comparing control/hypoxia offspring fed the high salt diet. Not significant (n.s.).
Back to article page