Figure 1
From: PDGF-BB enhances collagen gel contraction through a PI3K-PLCγ-PKC-cofilin pathway
PI3K or PLCγ inhibition reduces PDGF-enhanced collagen gel contraction. (A) Collagen gel contraction by BJ fibroblasts were studied in presence or absence of 20 ng/mL PDGF-BB. The line graph (Left) presents the dynamic of contraction during 24 h, and bar chart (Right) presents the contraction after 2 h to illustrate the early effect of PDGF-BB on contraction. (B,C) BJ fibroblast cells were treated with the PI3K inhibitor LY294002 (B) or the PLCγ inhibitor U73122 (C) and contraction proceeded in presence or absence of 20 ng/mL PDGF-BB. In all panels, values represent average of at least four independent experiments. (*) indicates p < 0.05, (**) indicates p < 0.01, and (***) indicates p < 0.001. Error bars are SEM.
