Figure 2
From: PDGF-BB enhances collagen gel contraction through a PI3K-PLCγ-PKC-cofilin pathway
PI3K and PLCγ are both required for PDGF-enhanced contraction. (A) PAE-Rβwt, (B) non-transfected PAE cells that lack PDGF-Rβ, and (C–J) eight PAE-Rβ mutants (listed in Table 1), were used in collagen gel contraction assay. Among the investigated mutant cells, only the PAE-Rβ Y740/751F and Y1009/1021F, the mutants that were unable to activate PI3K and PLCγ respectively, were unable to respond to PDGF stimulation by an enhanced contraction. PDGF-stimulation had no effect on non-transfected PAE cells, lacking PDGF receptors. In contrast, the PAE-Rβwt (panel A) and the other six PAE-Rβ mutants (panels D–I) showed enhanced contraction in response to PDGF-BB. In all panels, the graph to the left presents the time-course of contraction up to 8 h and the bar chart to the right side presents the contraction after 2 h. Values are averages of a minimum of four independent experiments, each performed in triplicate. Error bars are SEM. (*) refers to p < 0.05.
